Coding
REINT-6N
Part:BBa_K1433017:Design
Designed by: Chaofan Zhang Group: iGEM14_ZJU-China (2014-10-09)
P-C-attL-rT-rT-attR-B-6N-gp35-T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 457
Illegal NheI site found at 654 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 928
Illegal XhoI site found at 1015 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 1567
Illegal NgoMIV site found at 1654
Illegal AgeI site found at 704 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 99
Illegal BsaI.rc site found at 357
Illegal BsaI.rc site found at 1762
Design Notes
no
Source
Derive from 2014 Distribution Kit and synthesis by Genewiz..
References
- Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
- Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.